Bioinformatics Questions
Protein-protein interaction analysis in functional proteomics is performed using various experimental and computational methods. Experimental methods include techniques such as yeast two-hybrid assays, co-immunoprecipitation, and affinity purification coupled with mass spectrometry. These methods help identify and validate physical interactions between proteins.
On the other hand, computational methods involve the use of bioinformatics tools and algorithms to predict protein-protein interactions based on sequence, structure, and evolutionary information. These methods include protein docking, homology modeling, and network-based approaches.
Overall, a combination of experimental and computational approaches is often employed in protein-protein interaction analysis to provide a comprehensive understanding of the complex protein interaction networks in cells.