Bioinformatics Questions
Metabolic pathway analysis in metabolomics is performed by integrating various data analysis techniques and tools. This involves the identification and quantification of metabolites using techniques such as mass spectrometry or nuclear magnetic resonance spectroscopy. The obtained metabolite data is then mapped onto known metabolic pathways using databases such as KEGG or MetaboAnalyst. Statistical and bioinformatics methods are applied to analyze the data, including pathway enrichment analysis, pathway topology analysis, and pathway visualization. These analyses help in understanding the interconnectedness of metabolites and their roles in biological processes, identifying key pathways associated with specific conditions or treatments, and generating hypotheses for further experimental validation.